Biuret test: Learn how to perform

Learn how to perform Biuret test to detect peptide bonds and proteins in a substance. Find out the principle, reagent preparation, observation, and importance of the Biuret reaction. Biuret is a compound formed by heating urea at 180 0 It is the result of the condensation of 2 molecules of urea. The peptide bonds in Biuret give a positive result for the test hence the reagent is named so. Biuret test is a general test for compounds (proteins and peptides) having two or more peptide (CO-NH) bonds. Histidine is the only amino acid that gives Biuret test positive. Principle: When biuret is treated with dilute copper sulfate in alkaline medium, a purple colored compound is ... The Biuret Test is a classic biochemical assay widely utilized to identify the presence of proteins in a given sample. This colorimetric test is based on a reaction between peptide bonds and copper ions, resulting in a distinctive color change. With its simplicity and effectiveness, the Biuret test for protein is a crucial method in biological, food, and chemical analysis laboratories. Principle of the Biuret Test The Biuret Test operates on the fundamental principle that peptide bonds in ... Principle Of Biuret Test The biuret method is a colorimetric technique specific for proteins and peptides. Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds. In other words, Biuret test is based on the reaction of the cupric ions Cu 2+ with peptide bonds in an alkaline solution.