Hplc: Principle The purification takes place in a

HPLC Principle The purification takes place in a separation column between a stationary and a mobile phase. The stationary phase is a granular material with very small porous particles in a separation column. The mobile phase, on the other hand, is a solvent or solvent mixture which is forced at high pressure through the separation column. Via a valve with a connected sample loop, i.e. a small tube or a capillary made of stainless steel, the sample is injected into the mobile phase flow from ... High-performance liquid chromatography, abbreviated as HPLC , is a chromatographic technique of great versatility and analytic power used in many aspects of drug manufacturing and research. It separates or identifies mixtures of substances into their components based on their molecular structure and composition. The other name for high-performance liquid chromatography is high-pressure liquid chromatography. HPLC , aka High-Performance Liquid Chromatography, is a separation technique in analytical chemistry used to identify, quantify and separate Read principles, advantages, and applications of High-Performance Liquid Chromatography- a technique for separating and quantifying components in a sample.