Learn about the technique of HPLC , which separates, identifies, and quantifies specific components in mixtures using high pressure pumps and columns. Find out how HPLC works, what types of columns and detectors are used, and what applications it has in various fields. High-performance liquid chromatography ( HPLC ) is a powerful analytical technique used in chemistry, biochemistry, and pharmaceutical sciences for the separation, identification, and quantification of chemical compounds in complex mixtures. What is HPLC (High-performance liquid chromatography )? High-Performance Liquid Chromatography ( HPLC ) is a sophisticated analytical technique used extensively in various scientific fields to separate, identify, and quantify the components of a mixture. This technique, originally known as high-pressure liquid chromatography , has evolved significantly since its inception. HPLC operates on the principle of column chromatography but utilizes advanced technology to enhance its performance. Unlike ... High-performance liquid chromatography ( HPLC ) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially …
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